1. The enzyme that accomplishes the unwinding of the original double stranded DNA molecule, once supercoiling has been eliminated, by breaking the hydrogen bonds that hold the two strands together is :   Topoisomerase   DNA Polymerase II   Primase   Helicase   Hint Half-n-half Clue   2. What does transformation involve in Bacteria ?   the infection of cells by a phage DNA molecule   the creation of a strand of RNA from DNA   assimilation of external DNA into a cell   the creation of a strand of DNA from RNA   Half-n-half Clue   3. The enzyme responsible for initiating the unwinding of double-stranded DNA (eliminating supercoiling) by nicking a single strand of the DNA molecule is :   Helicase   Ligase   Topoisomerase   Gyrase   Half-n-half Clue   4. An enzyme that recognizes and cuts DNA only at a particular sequence of nucleotides is often called :   Restriction endonuclease   RNA polymerase   Photolyase   DNA glycosylase   Half-n-half Clue   5. A major difference between DNA replication in prokaryotes and eukaryotes is :   DNA amylase performs the function of DNA helicase in prokaryotes   there is only one replication origin in prokaryotes.   replication is conservative in prokaryotes   prokaryotes do not use Topoisomerase in the replication process   Half-n-half Clue   6. A mamalian cell typically has 1.2 meters (when completely outstretched) of double stranded DNA.The total time to duplicate the DNA is 5 hours. How many origins of replication are there if the rate of duplication is 16µmeters/min ?   250   15000   1   500   Half-n-half Clue   7. The enzyme X removes RNA primers attached by Primase and this gap is then filled in by DNA Polymerase I. The enzyme X is :   RNase H   DNA amylase   DNA ligase   Reverse Transcriptase   Half-n-half Clue